human acyl ghrelin  (Tocris)

Journal: Journal of psychiatric research

Article Title: Ghrelin is associated with an elevated mood after an overnight fast in depression.

doi: 10.1016/j.jpsychires.2024.04.053

Figure Lengend Snippet: Fig. 1. Fasting levels of acyl ghrelin were not associated with metabolic state ratings. A: To investigate the relationship between plasma levels of ghrelin and subjective metabolic and mood state, 103 participants, comprising 52 HCP and 51 patients with MDD, were invited after a 12-h overnight fast to the laboratory. Following a blood draw, participants indicated their current subjective metabolic state and their mood on 24 Visual analog scales (VAS). Additionally, they completed various questionnaires to assess their trait anxiety and depression subtype. B–D: After log-transforming and residualizing for age, sex, and BMI fasting levels of acyl ghrelin were not associated with subjective ratings of hunger (HCP: r = −0.06, p = 0.69; MDD: r = −0.052, p = 0.72), satiety (HCP: r = 0.11, p = 0.48; MDD: r = 0.14, p = 0.34), and thirst (HCP: r = 0.075, p = 0.62; MDD: r = 0.035, p = 0.81) in HCP and MDD. For des-acyl ghrelin there were also no correlations with these metabolic state items (Fig. S2).

Article Snippet: Cryo tubes containing plasma and HCl were analyzed using the acylated ghrelin (human) easy sampling ELISA kit #A05306 and the unacylated ghrelin (human) easy sampling ELISA kit #A05319 from Bertin Bioreagent (Bertin Technologies, 10 bis Avenue Ampère, Parc d’Activités du Pas du Lac, 78180 Montigny-le-Bretonneux, France; distributed by BioCat GmbH, Germany).

Techniques: Clinical Proteomics

Journal: Journal of psychiatric research

Article Title: Ghrelin is associated with an elevated mood after an overnight fast in depression.

doi: 10.1016/j.jpsychires.2024.04.053

Figure Lengend Snippet: Fig. 2. Higher levels of acyl ghrelin are associated with an elevated mood. A–C: Participants with higher levels of acyl ghrelin after fasting show a higher mood state (r = 0.25, p = 0.012), a higher positive affect (r = 0.21, p = 0.041), and less negative affect (r = −0.22, p = 0.031). D: When focusing on patients with MDD, the correlation between levels of acyl ghrelin and mood state was strongest in patients without any medication (r = 0.33, p = 0.091). Depicted are bootstrapped correlation estimates (B = 2000). E: For positive mood alone, we did not find differences across groups. F: For negative mood alone, acyl ghrelin showed stronger associations in patients without medication (r = −0.44, p = 0.021) in comparison to patients taking SSRI (r = 0.12, p = 0.69) or other antidepressants (r = −0.17, p = 0.64). No AD = no antidepressive medication, SSRI = selective serotonin reuptake inhibitors, Other = non-SSRI medication.

Article Snippet: Cryo tubes containing plasma and HCl were analyzed using the acylated ghrelin (human) easy sampling ELISA kit #A05306 and the unacylated ghrelin (human) easy sampling ELISA kit #A05319 from Bertin Bioreagent (Bertin Technologies, 10 bis Avenue Ampère, Parc d’Activités du Pas du Lac, 78180 Montigny-le-Bretonneux, France; distributed by BioCat GmbH, Germany).

Techniques: Comparison

Journal: Journal of psychiatric research

Article Title: Ghrelin is associated with an elevated mood after an overnight fast in depression.

doi: 10.1016/j.jpsychires.2024.04.053

Figure Lengend Snippet: Fig. 3. The association between fasting levels of acyl ghrelin and mood is not specific to items, but attributable to overall mood. A: We found negative correlations between levels of acyl ghrelin and ratings across all items of negative affect, particularly in patients with MDD with the strongest association for the item “afraid” (r = −0.34, p = 0.015). B: There were positive correlations between levels of acyl ghrelin and overall positive affect as well as ratings across all items of positive mood. For patients with MDD, the strongest association was observed for the item “active” (r = 0.26, p = 0.071). C: To investigate if the association between acyl ghrelin and mood is driven by specific items, we extracted a common factor and recalculated the original correlations for each mood item (residualized items). Our analysis shows that the link between acyl ghrelin and mood is not item-specific. The broken line depicts the association with mood state the unrotated factor, demonstrating that single items do not exceed this association.

Article Snippet: Cryo tubes containing plasma and HCl were analyzed using the acylated ghrelin (human) easy sampling ELISA kit #A05306 and the unacylated ghrelin (human) easy sampling ELISA kit #A05319 from Bertin Bioreagent (Bertin Technologies, 10 bis Avenue Ampère, Parc d’Activités du Pas du Lac, 78180 Montigny-le-Bretonneux, France; distributed by BioCat GmbH, Germany).

Techniques:

Journal: Journal of psychiatric research

Article Title: Ghrelin is associated with an elevated mood after an overnight fast in depression.

doi: 10.1016/j.jpsychires.2024.04.053

Figure Lengend Snippet: Fig. 4. Fasting levels of acyl ghrelin are more strongly associated with state versus trait-like markers of negative affectivity. A: To measure negative affectivity, we calculated the depression and anxiety subscores of the STAI-T. Scores of the STAI-T were strongly correlated with negative mood ratings. B: Scores of the STAI-T were moderately correlated with positive mood ratings. C: Acyl ghrelin was not associated with STAI-T scores (overall STAI-T: r = −0.15, p = 0.15). No correlations between STAI-T sub-scores and levels of acyl and des-acyl ghrelin.

Article Snippet: Cryo tubes containing plasma and HCl were analyzed using the acylated ghrelin (human) easy sampling ELISA kit #A05306 and the unacylated ghrelin (human) easy sampling ELISA kit #A05319 from Bertin Bioreagent (Bertin Technologies, 10 bis Avenue Ampère, Parc d’Activités du Pas du Lac, 78180 Montigny-le-Bretonneux, France; distributed by BioCat GmbH, Germany).

Techniques: